Comparative detection of coliform organisms in milk and water by the presumptive coliform test

Coliform Organisms in Certified Milk.

Detection of coliform organisms in drinking water by radiometric method.

The radiometric method has been used for detection of coliform bacteria in water. The method is based on measuring the released metabolic 14CO2 from 14C-lactose in growth media containing coliform organisms incubated at 37 degrees C under continuous shaking. This rapid and sensitive radiometric method permits the detection of even single coliform organisms within 6 hours of incubation. Using th...

Radiometric method for the detection of coliform organisms in water.

A new radiometric method for the detection of coliform bacteria in water has been described. The method is based on the release of (14)CO(2) from [(14)C]lactose by bacteria suspended in growth medium and incubated at 37 C. The evolved (14)CO(2) is trapped by hyamine hydroxide and counted in a liquid scintillation spectrometer. The method permits the detection of 1 to 10 organisms within 6 h of ...

Optimum membrane structures for growth of coliform and fecal coliform organisms.

The purpose of this study was to determine the optimum membrane filter structure and characteristics for recovery of coliform organisms. Additionally, other factors such as sterilization method and membrane composition were examined. Fecal coliform growth tests with varied samples indicated that the most critical factor in recovery was surface pore morphology and not other factors previously su...

Significance of the presumptive coliform test as applied to orange juice.

The presumptive test for the determination of coliforms in food products is based on the inoculation of tubes of lactose broth with specified amounts of the material (A.P.H.A., 1946). Fermentation of the sugar with formation of gas indicates a positive presumptive test for coliforms. This test was originally developed for the examination of water and milk samples, but in recent years it has bee...